split method is the CloudFormation Fn::Split equivalent.
resource("Instance", "AWS::EC2::Instance", InstanceType: ref("InstanceType"), ImageId: "ami-0de53d8956e8dcf80", SecurityGroupIds: split(",", ref("SecurityGroups")) )
Resources: Instance: Type: AWS::EC2::Instance Properties: InstanceType: Ref: InstanceType ImageId: ami-0de53d8956e8dcf80 SecurityGroupIds: Fn::Split: - "," - Ref: SecurityGroups
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